Metabolic disorders on the horizon with PamGene technology
Researchers from Professor Fajas' group at the University of Lausanne, Switzerland, and their collaborators, have revealed novel and exciting insights about how CDK4 participates in adipose tissue biology and in insulin signalling.
|Insulin signalling is very important in controlling metabolic processes that regulate the energy needs of cell function. Therefore defects in this signalling contributes to insulin resistance, which is a complication of various metabolic disorders including obesity and type2 diabetes. Exact mechanisms and underlying pathways are not very well understood. Initial clues that the cyclin-dpendent kinase 4 (CDK4), known to regulate cell cycle, could play a role in adipose tissue biology came from findings that it was overexpressed in white adipose tissue (WAT). In CDK4-deficient mice, lipogenesis was impaired, and lipolysis was increased, and reverse when CDK4 was overexpressed. Global kinome analysis using the PamGene platform, in CDK4-defecient mice after insulin stimulation showed defective insulin signalling. Further, a putative role of CDK4 upstream of AKT in the insulin signalling pathway was proposed.
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Differences in putative kinase activities between control and Cdk4ncmice injected with insulin for 3 minutes
|CDK4 is an essential insulin effector in adipocytes.
Lagarrigue S, Lopez-Mejia IC, Denechaud PD, Escoté X, Castillo-Armengol J, Jimenez V, Chavey C, Giralt A, Lai Q, Zhang L, Martinez-Carreres L, Delacuisine B, Annicotte J-S, Blanchet E, Huré S, Abella A, Tinahones FJ, Vendrell J, Dubus P, Bosch F, Kahn RC and Fajas L. (2015) J Clin Invest. 2015. doi:10.1172/JCI81480.
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