UABs Kinome Core scores a hatrick with 3 new publications
UABs Kinome Core scores a hatrick with 3 new publications involving PamGenes kinase activity profiling platform!
Kinomic profiling of electromagnetic navigational bronchoscopy specimens: a new approach for personalized medicine.
Anderson JC, Minnich DJ, Dobelbower MC, Denton AJ, Dussaq AM, Gilbert AN, Rohrbach TD, Arafat W, Welaya K, Bonner JA, Willey CD. PLoS One. (2014) Dec 30;9(12):e116388
  The protective effect of p16INK4a in oral cavity carcinomas: p16Ink4A dampens tumor invasion-integrated analysis of expression and kinomics pathways.
Isayeva T, Xu J, Ragin C, Dai Q, Cooper T, Carroll W, Dayan D, Vered M, Wenig B, Rosenthal E, Grizzle W, Anderson J, Willey CD, Yang ES, Brandwein-Gensler M. Mod Pathol. 2014 Dec 19. 
  Development of a Sox2 reporter system modeling cellular heterogeneity in glioma.
Stoltz K, Sinyuk M, Hale JS, Wu Q, Otvos B, Walker K, Vasanji A, Rich JN, Hjelmeland AB, Lathia JD. Neuro Oncol. 2014 Nov 21. pii: nou320. [Epub ahead of print]

 
 
 
 
   
Researchers tackle 2 challenges in finding relevant Lung cancer biomarkers: that of insufficent biopsy material, and a molecular test to directly measure kinases in the tumour biopsies. They combined a novel minimally invasive biopsy technique that expands the number of lesions amenable for biopsy with subsequent ex vivo kinase activity analysis using PamGene’s platform. They could identify patient-specific as well as drug-specific kinase activity profiles from small quantities of tissues, establishing a method for the future study of biomarkers for precision medicine.   It is documented that many HPV-mediated oropharyngeal squamous cell carcinomas (OPSCCs) are are associated with improved outcome compared with HPV-negative counterparts. Improved survival and increased radiosensitivity is also associated with  p16INK4a overexpression. However underlying mechanisms remain elusive, and this study sheds some light with an integrated approach using invasive assays, kinomics profiling, gene expression and pathway analysis. Different p16INK4a-sensitive pathways with two potential mechanisms were revealed, one with increased EGFR activity and STAT3 activation and another with decreased PDGFR and STAT3 inactivation. Their data suggested that irrespective of transcriptionally active HPV status, p16INK4a overexpression is an inhibitor of invasion for head and neck squamous cell carcinoma.   Gliomas are difficult to treat partly due to intra-tumour heterogeneity, associated with tumour-initating cells (TICs).  These cells increase tumorigenic potential and can survive both chemo and radio therapies and drive recurrence. Sox2 is often overexpressed in gliomas, and targeting it could decrease GBM cell invasion and migration. The researchers generated and implanted Sox2-GFP-high cells in mice to generate tumours containg both high and low Sox2. Kinomic analysis of FACS-sorted cells revealed important differences in the two types and identified active feline sarcoma (Fes) signaling in Sox2–EGFP-high cells. This model could empower studies on cellular hetrogeneity and is useful to test preclinical treatment efficiency.