Tyrosine Kinase PamChip

Tyrosine Kinase PamChip® Array for PamStation®12 consists of 144 peptides* with known phosphorylation sites, representing 100 different proteins. Peptide sequences are derived from literature or computational predictions and are correlated with one or multiple upstream kinases.
 

* In October 2017, PamGene has released an expanded version with 196 peptides. 

 

 

PamChip Content, Protein Tyrosine Kinase Arrays

 

PamChip 86312 (144 peptides)

   

PamChip 86402 (196 peptides)

   

 

 

Additional Information

 

 

 

 

PamChip Assays

Detailed information and documents for kinase assays can be accessed with PamCloud Login.

 

 

PamChip PTK Assay Principle

Kinase(s) in the sample (recombinant, cell or tissue lysate) actively phosphorylate substrates on the PamChip®, in the presence of ATP. A FITC-conjugated PY20 antibody, present in the reaction mix is used to detect the phosphorylated Tyr to quantify the phosphorylation signal. Images are taken every 5 minutes to generate real time kinetics data.