Serine / Threonine Kinase PamChip® Array for PamStation®12 consists of 140 ser/thr containing peptides and 4 positive control phosphorylated peptides resulting in 144 peptides per array. Each peptide represents a 15-amino-acid sequence of which 13 residues are derived from a putative phosphorylation site in human proteins. Peptide sequences are derived from literature and are correlated with one or multiple upstream kinases.
PamChip Content, Serine/Threonine Kinase Arrays
STK PamChip 87102
Detailed information and documents for kinase assays can be accessed with PamCloud Login.
PamChip STK Assay Principle
Kinase(s) in the sample (recombinant, cell or tissue lysate) actively phosphorylate substrates on the PamChip®, in the presence of ATP. An antibody mix is used to detect the phosphorylated Ser/Thr, and a 2nd FITC-conjugated antibody is used in a detection mix to quantify the phosphorylation signal.