PamGene's 3D Microarray Technology is ideal for Kinase Profiling. You can test your kinase on a large panel of kinase substrates and get real-time kinetic read-out giving you simultaneous:

IC50 Results

Selectivity Data

Mechanism of Action information

Multiple Rate Constants

Using the PamStation^®4 (4 arrays per chip) or the PamStation^®96 (96 arrays per chip) together with PamChip^® Arrays offers significant benefits for Kinase Substrate Profiling and Kinase Inhibitor Screening including:

1) Multiplex Analysis

Many kinase activities can be monitored in one experiment unlike most current technologies that permit analysis of only one kinase per experiment. It is therefore possible to:

- investigate the substrate profiles of 4 or 96 kinases at once, depending on your throughput requirements
- monitor the effects of 4 or 96 kinase inhibitors
- monitor the effects of different kinase inhibitor concentrations in one single experiment

2) Initial Velocity Measurements

The unique ability of PamGene's technology to generate kinetic read-out enables initial velocity measurements of enzyme activity to be made. Initial velocity data measures the conversion of substrate to product during the linear phase and therefore generates the most accurate and statistically significant data on enzyme activity

3) Spotting of Peptide Substrates at Varying Concentrations

The 3-Dimensional nature of the PamChip^® Array surface enables peptides to be spotted at low and high concentrations. The ability to study kinase inhibitors at different peptide substrate concentrations is vital for determining their mechanism of action. Because different peptide substrate concentrations can be spotted within one well, data generation is rapid.

4) Potency Measurements

Multiple inhibitor concentrations can be analysed in parallel, allowing IC50 values and inhibitor potency to be determined

5) Selectivity Screening

Since most kinases are promiscuous, determination of inhibitor selectivity is a major issue. Assay conditions such as ATP, kinase and inhibitor concentration can be quickly and easily changed while using multiple different kinases. Put together with initial velocity data, valuable information on selectivity can thus be obtained.

6) Simultaneous Analysis of Data

Mechanism of action, IC50 values, potency, specificity data and off-target effects can all be measured simultaneously, in one experiment


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