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In addition to the kinase application used in our Matching Medicines approach, our technology can used in a range of other applications.
PamGene has a series of Application Notes outlining a number of applications in more detail. The following are available:
Transcript Profiling
Mutation Detection (x2)
Genotyping
Assay development for High Throughput Screening using PamChip® Microarrays
Simultaneous potency determination and selectivity profiling of Erb-B4 kinase inhibitors
Multiplex kinase assay for selectivity profiling and potency determination of kinase inhibitors (examining multiple kinases in one well)
Detection of tumour cell lysate kinase activity from frozen tissue slices on PamChip® Microarrays.
For a copy of any of these application notes, email
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| Gene Expression Profiling |
A number of projects on gene expression profiling will involve using the technique for the investigation of bone and cartilage pathogenesis, asthma and colorectal cancer. The aim is to use abbreviated gene expression profiles (lower biological content than conventional gene expression microarrays) for predictive toxicology and therapeutic efficacy.
A parallel project utilizing a proprietary mRNA amplification technique called TYRAS is being developed to tackle inherent weaknesses in the Eberwine procedure when low amounts of starting material are used. The necessary amount of replication cycles decreases the representation of the low abundance messenger RNA population considerably in the Eberwine reaction. We aim to develop TYRAS as an alternative approach with better representation of the original material after amplification. The absence of intermediary DNA in the TYRAS approach in combination with a much less laborious protocol may prove to be the essential factors to making this a viable alternative for gene expression studies.
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